Vis-NIR reflectance spectrum of DNA at 293±2 K
- Title
- Vis-NIR reflectance spectrum of DNA at 293±2 K
- DOI
- 10.26302/SSHADE/EXPERIMENT_OP_20180111_001
- Data reference
- Poch, Olivier (2014): Vis-NIR reflectance spectra of biomolecules dry powders at 293±2 K. SSHADE/BYPASS (OSUG Data Center). Dataset/Spectral Data. https://doi.org/10.26302/SSHADE/EXPERIMENT_OP_20180111_001
- Publications
- Comments
- Spectrum published in Figure 2 of the publication.
- Database
- Experimentalists
- Experiment type
- laboratory measurement
- Type
- reflectance factor
- Comments
- In the reduced data set, absolute uncertainty on the calibrated reflectance factor is ±0.1, and the spatial variability of the reflectance across the field of view is of the order of 10%. Thus, the SCITEAS imaging system does not provide an accurate measurement of the absolute reflectance, but it provides a spectrum resolved in wavelength from 0.4 to 2.4 μm.
- Instrument
- SCITEAS Imaging System - hyperspectral imaging system Vis-NIR
- Sample holder
- The SCITEAS chamber was fully open, at room temperature and pressure. The samples were deposited on top of a big aluminum cylinder, directly below the imaging system (no window).
- Standard medium
- air
- Observation mode
- spectrum
- Spectral range type(s)
- Vis, NIR
- Valid spectral range(s)
-
Min - Max (${\mu}m$) Sampling (${\mu}m$) Resolution (${\mu}m$) Position accuracy (${\mu}m$) Absorption edge #1 0.38 - 0.96 0.02 0.015 #2 0.96 - 2.4 0.01 0.03 - Scan number
- 5
Definition: incidence and emergence angles are positive with origin at nadir, and vary in same direction. Azimuth origin (increasing clockwise) is for i = e (opposition geometry).
- Observation geometry
- biconical
- Observation mode
- fixed angles
- Emergence angle
- 13.0°
- Azimuth angle
- 0.0°
- Comments
- The light beam emitted from the extremity of the fibre bundle positionned about 300 mm above the sample surface has a divergence half-angle of 12.7°. The light source incidence and azimuth angles are slightly variable from pixel to pixel.
Illumination
Observation
- Comments
- partial polarization variable with wavelength (from monochromator grating)
- Observation mode
- roi averaged
- Name
- Deoxyribonucleic acid sodium salt (DNA)
- Primary material(s)
- Primary constituent(s)
- Experiment
- Vis-NIR reflectance spectra of biomolecules dry powders at 293±2 K
- Date begin
- 2014-02-13
- Release date
- 2018-01-31 23:51:01 UTC+0000
- Version (Date)
- #1 (2018-01-31 23:51:01 UTC+0000)
- History
-
Date Mode Version Status Comments 2018-01-20 23:09:59 UTC+0000 first import #1 valid version new reflectance spectrum
- Analysis
- White Spectralon (99%) plates (Labsphere) were used as external and internal calibration standards.
- Quality flag
- 4
