- Names
-
- Perrine Chaurand
- Wei Liu
- Daniel Borschneck
- Clement Levard
- Melanie Auffan
- Emmanuel Paul
- Blanche Collin
- Isabelle Kieffer
- Sophie Lanone
- Jerome Rose
- Jeanne Perrin
- Title
- Multi-scale X-ray computed tomography to detect and localize metal-based nanomaterials in lung tissues of in vivo exposed mice
- Abstract
- In this methodological study, we demonstrated the relevance of 3D imaging performed at various scales for the ex vivo detection and location of cerium oxide nanomaterials (CeO2-NMs) in mouse lung. X-ray micro-computed tomography (micro-CT) with a voxel size from 14 microns to 1 micron (micro-CT) was combined with X-ray nano-computed tomography with a voxel size of 63 nm (nano-CT). An optimized protocol was proposed to facilitate the sample preparation, to minimize the experimental artifacts and to optimize the contrast of soft tissues exposed to metal-based nanomaterials (NMs). 3D imaging of the NMs biodistribution in lung tissues was consolidated by combining a vast variety of techniques in a correlative approach: histological observations, 2D chemical mapping and speciation analysis were performed for an unambiguous detection of NMs. This original methodological approach was developed following a worst-case scenario of exposure, i.e. high dose of exposure with administration via intra-tracheal instillation. Results highlighted both (i) the non-uniform distribution of CeO2-NMs within the entire lung lobe (using large field-of-view micro-CT) and (ii) the detection of CeO2-NMs down to the individual cell scale, e.g. macrophage scale (using nano-CT with a voxel size of 63 nm).
- Keywords
- Ce, Nano-particles, XANES, x-ray absorption spectroscopy
- Content
- sample, spectral data, spectral data use
- Year
- 2018
- Journal
- Scientific Reports
- Volume
- 8
- Number
- 1
- Pages
- 4408
- Document type
- article
- Publication state
- published
- Comments
- None
- Doi
- 10.1038/s41598-018-21862-4
- Identifiers
-
- URL: http://dx.doi.org/10.1038/s41598-018-21862-4
- bibcode: